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KMID : 0357920020360010021
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Korean Journal of Pathology
2002 Volume.36 No. 1 p.21 ~ p.29
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Altered Expression of Nephrin, Glomerular Epithelial Cell Protein-1, (GLEPP1) and WT-1 in Glomerular Disease
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Kim Byoung-Kwon
Kim Ji-Hoon
Lee Hyun-Soon
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Abstract
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Background: Glomerular epithelial cell protein-1 (GLEPP1)and WT-1 expressed in mature visceral glomerular epithelial cell(VGEC)is required for maintenance of the mature status of VGEC. Nephrin protein is located at the filtration slit and regarded as a molecular component of the slit diaphragm. Alterations of these proteins in proteinuric diseases are not clearlydefined.
Methods: We investigated the expression of GLEPP1, WT-1 and nephrin in 28 renal biopsies diagnosed with minimal change nephropathy (n=10), focal glomerulosclerosis(n=10) and membranous nephritis (n=8) by immunohistochemical staining. Normal control biopsies were obtained from six nephrectomy specimens.
Results: The patients consisted of 15 males and 13 females. The mean age was 40.7 years. Nephrotic range proteinuria (≥3.5 g/day) was noted in 15(54%) patients. GLEPP1 and nephrin expression were significantly decreased in patients as compared with those of the controls (p＜0.05). The mean number of WT-1 expressing cells per glomerulus was also significantly decreased in patients as compared with those of the controls (p＜0.05). However, there was no significant difference in the number of WT-1 expressing cells among the disease groups.
Conclusions: These results suggest that the loss of biological markers of mature VGEC may play an important role in the pathogenesis of proteinuria.
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KEYWORD
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Nephrosis, Lipoid, Glomerulo Nephritis, Biological Markers
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